Monday, 21 July 2014

Order of incubation for recovery of bacteria and fungi


One of the dilemmas for the environmental monitoring of controlled environments, when a single culture medium is used, is with the order and length of incubation. The two most common regimes adopted are either 20-25oC followed by 30-35oC and 30-35oC followed by 20-25oC. There have been very few published studies to help the microbiologist with the selection of the optimal incubation regime.

Tim Sandle has written a peer-reviewed research article for the International Journal of Pharmaceutical Compounding. In the article, Dr. Sandle presents experimental data which examines the optimal order of incubation and compares recovery rates for bacteria and fungi.

The conclusion of the study is that the incubation regime has an impact on the numbers of microorganisms recovered and the incidence of recovery. Specifically, where the recovery of fungi is important, the research indicates that the 20-25oC followed by 30-35oC is superior.



Sandle, T. (2014) Examination of the Order of Incubation for the Recovery of Bacteria and Fungi from Pharmaceutical Cleanrooms, International Journal of Pharmaceutical Compounding, 18 (3): 242 – 247
 Posted by Tim Sandle

Posted by Tim Sandle