Sunday, 1 March 2015

Culture reference strains

Examples of reference strains

Here is a handy list of reference strains for different microbiological tests.

Test
Example Reference Strain
Aesculin Hydrolysis Test
Positive control
Negative control
Enterococcus faecalis NCTC 12697
Streptococcus agalactiae NCTC 8181
Agglutination Test
Positive control
Negative control
N/A
Bile Solubility Test
Positive control
Negative control
Streptococcus pneumoniae NCTC 12977
Streptococcus mitis NCTC 10712
Catalase Test
Positive control
Negative control
Staphylococcus aureus NCTC 6571
Streptococcus mitis NCTC 10712
Coagulase Test
Positive control
Negative control
Staphylococcus aureus NCTC 6571
Staphylococcus haemolyticus NCTC 11042
Deoxyribonuclease Test
Positive control
Negative control
Staphylococcus aureus NCTC 6571
Staphylococcus haemolyticus NCTC 11042
Indole Test
Positive control
Negative control
Escherichia coli NCTC 10418
Proteus mirabilis NCTC 10975
Motility Test
Positive control
Negative control
Proteus mirabilis NCTC 10975
Acinetobacter lwoffii NCTC 5866
Nagler Test
Positive control
Negative control
Clostridium perfringens NCTC 8359*
Clostridium difficile NCTC 11204*
ONPG (ß-Galactosidase) Test (for
Enterobacteriaceae)
Positive control
Negative control
Escherichia coli NCTC 10418
Proteus mirabilis NCTC 10975
ONPG (ß-Galactosidase) Test (for Neisseria species
Positive control
Negative control
Neisseria lactamica NCTC 10617
Neisseria gonorrhoeae NCTC 8375
Optochin Test
Positive control
Negative control
Streptococcus pneumoniae NCTC 12977
Streptococcus mitis NCTC 10712
Oxidase Test
Positive control
Negative control
Pseudomonas aeruginosa NCTC 10662
Escherichia coli NCTC 10418
Oxidation/Fermentation of Glucose Test
(Gram negative rods)
Oxidation:
Positive control
Negative control
Pseudomonas aeruginosa NCTC 10662
Acinetobacter Iwoffii NCTC 5866
Oxidation/Fermentation of Glucose Test
(Gram positive cocci)
Oxidation:
Positive control
Negative control
Micrococcus luteus NCTC 2665
OF basal medium without carbohydrate
Porphyrin synthesis (ALA) Test
Positive control
Negative control
Haemophilus parainfluenzae NCTC 10665
Haemophilus influenzae NCTC 11931
Potassium Hydroxide Test
Positive control
Negative control
Escherichia coli NCTC 10418
Staphylococcus aureus NCTC 6571
Changing the Phase of Salmonella
Positive control
Negative control
N/A
Thermonuclease Test
Positive control
Negative control
Staphylococcus aureus NCTC 6571
Staphylococcus haemolyticus NCTC 11042
Urease Test
Positive control
Negative control
Proteus mirabilis NCTC 10975
Escherichia coli NCTC 10418
X and V factor Test
X and V factor
Haemophilus influenzae NCTC 11931
V factor only
Haemophilus parainfluenzae NCTC 10665
X factor only
Haemophilus haemoglobinophilus NCTC 8540*


Source: Public health England

Posted by Tim Sandle