Saturday, 28 April 2018

Rapid test to select the correct antibiotic


Standard practices for the infectious diagnostics require up to 72 hours to allow for a reliable result. This is due to the fact, that the number of pathogens in a patients sample is too small to conduct tests. Analysis is therefore only possible after time-consuming cultivation. Especially in clinical application during treatments of severe infections e.g. a sepsis time is a crucial factor. Intensive physicians are confronted with an alarming dilemma: "far too often we have to administer broad-spectrum antibiotics 'blindly', because we can neither analyse pathogen nor potential resistances. Therefore, we possibly use a sledge-hammer to crack a nut. A vicious cycle that aides the development of new resistances," explains Prof. Michael Bauer, director of the Clinic of Anesthesiology and Intensive Care at the University Hospital Jena.

The new method out of Jena provides much faster diagnosis as basis for a decision of a reliable therapy. Ute Neugebauer, who works at Leibniz-IPHT and the University Hospital Jena points to tiny electrodes that are fixed on the surface of a stamp-sized chip. The combination of fast, light-based diagnostics and a high automation level reduces the time from sampling to result from to date 72 to three and a half hours.

See:

Johanna Kirchhoff, Uwe Glaser, Jürgen A. Bohnert, Mathias W. Pletz, Jürgen Popp, Ute Neugebauer. Simple Ciprofloxacin Resistance Test and Determination of Minimal Inhibitory Concentration within 2 h Using Raman SpectroscopyAnalytical Chemistry, 2018; 90 (3): 1811 DOI: 10.1021/acs.analchem.7b03800

Posted by Dr. Tim Sandle

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