Insight into pathogenic bacterium Pseudomonas aeruginosa

Researchers have identified how the pathogenic bacterium Pseudomonas aeruginosa uses tension-activated membrane channels to stop itself from swelling up and bursting when it is suddenly exposed to water. The study helps explain how this bacterium -- a major cause of hospital-acquired infections -- persists in a variety of different environments.

P. aeruginosa is a remarkably adaptable bacterium. It naturally occurs in soil and freshwater, but it can also thrive on the moist surfaces of medical equipment, leading to opportunistic infections of immunocompromised or otherwise vulnerable patients that result in life-threatening conditions like pneumonia or sepsis.

One reason for P. aeruginosa's adaptability is its capacity to survive sudden changes in the water content of its environment. Rainfall, for example, poses a problem for bacteria because it dilutes the cell's surrounding media, prompting water to enter the cell by osmosis. Such "osmotic downshocks" can cause cells to swell up and burst, but some bacteria, including the gut bacterium Escherichia coli, carry mechanosensitive channels in their cell membranes that open up as soon as the cell starts to swell, letting small solute molecules -- or osmolytes -- exit the cell so that the osmotic gradient driving water influx is reduced. E. coli carries two types of mechanosensitive channels. The MscS channel releases modest amounts of osmolytes when swelling causes low amounts of membrane tension. The MscL channel is activated at higher tension levels to release osmolytes in larger amounts.

The researchers determined that P. aeruginosa expresses one MscL- and two MscS-type channels. The MscL-type channel predominates and is present at a higher density in P. aeruginosa membranes than its equivalent in E. coli membranes, allowing P. aeruginosa cells to release larger amounts of osmolytes. But the two MscS-type channels are also important; they are activated earlier in response to cell swelling, and, by releasing modest amounts of osmolytes, they can reduce membrane tension and prevent the MscL channels from releasing more osmolytes than necessary.

See:

Uğur Çetiner, Ian Rowe, Anthony Schams, Christina Mayhew, Deanna Rubin, Andriy Anishkin, Sergei Sukharev. Tension-activated channels in the mechanism of osmotic fitness in Pseudomonas aeruginosa. The Journal of General Physiology, 2017; jgp.201611699 DOI: 10.1085/jgp.201611699

Posted by Dr. Tim Sandle