Several developments took place in the field recently. A team of scientists from the University of Chicago Medicine (UChicago) came up with a novel high-throughput RNA sequencing strategy with the aim of studying the gut microbiome. Researchers at the Stanford lab of Stephen Quake are adopting a single cell RNA sequencing (scRNA-seq) in order to provide insights into food allergies and antibodies that cause them. QIAGEN, a Netherlands-based provider of sample and assay technologies for molecular diagnostics, launched a technology which allows faster and simpler library preparation for RNA sequencing.
UChicago Scientists Develop Revolutionary Strategy
Researchers at the University of Chicago recently discovered an RNA sequencing strategy for studying the activity of the gut microbiome. The strategy allows scientists to understand how tRNA changes dynamically within microbiomes and gives insights into how naturally occurring microbiomes respond to environmental changes such as temperature variation or changes in nutrient availability. The team of scientists created new tools to study transfer RNA (tRNA) in mouse gut microbiomes. According to the study, tRNA sequencing was applied to samples from the gut microbiome of mice that were on a high-fat or low-fat diet. New tools were employed to create a set of tRNA molecules from the samples. The bacteria from where the tRNA molecules originated were tracked and the post-transcriptional modifications that occurred were identified and measured. The tools used can identify two modifications in a high-throughput sequencing and analysis workflow. The level of one of the modifications was found increased in gut microbiomes of mice in a high-fat diet. For the first time scientists could notice a change in the modification level in tRNA in any microbiome.
QIAGEN Introduces a Breakthrough Technology for RNA Sequencing
QIAGEN unveiled a novel technology which facilitates faster and simpler library preparation for RNA sequencing. A prime component of this technology, the QIAseq FastSelect RNA Removal Kit allows scientists to target the types of RNA that are unrelated to their research and eliminate them from RNA-seq libraries for next-generation sequencing (NGS). According to Dr. Thomas Schweins, Senior Vice President of QIAGEN’s Life Sciences Business Area, the QIAseq FastSelect RNA Removal Kit enables faster removal of RNA types from a given sample, thus allowing scientists to achieve quality and reproducible RNA sequencing results. It also helps reduce time and cost. The kit helps to simplify and accelerate the process of RNA removal.
Posted by Dr. Tim Sandle, Pharmaceutical Microbiology
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