The
Columbia team behind the revolutionary 3D SCAPE microscope announces today a
new version of this high-speed imaging technology. In collaboration with
scientists from around the world, they used SCAPE 2.0 to reveal previously
unseen details of living creatures -- from neurons firing inside a wriggling
worm to the 3D dynamics of the beating heart of a fish embryo, with far
superior resolution and at speeds up to 30 times faster than their original
demonstration.
These improvements
to SCAPE
promise to impact fields as wide ranging as genetics, cardiology and
neuroscience.
SCAPE
2.0's improved resolution also enabled the team to image samples created using
tissue clearing and tissue expansion. These methods let scientists see
structures and connections deep inside intact samples, from whole mouse brains
to tumors and human biopsies. Although these samples are not alive, they are
very large and take a long time to image using standard microscopes. Today's
paper demonstrates that SCAPE 2.0 could image these types of samples at
record-breaking speeds. The researchers worked with Lambert Instruments,
leveraging the company's ultra-fast HiCAM Fluo camera. This camera was used to
capture images at more than 18,000 frames per second in the zebrafish embryo's
beating heart. This new configuration opened the door to recording individual
neurons firing in a freely moving C. elegans worms, giving the first view of an
animal's complete nervous system in action. SCAPE 2.0's other upgrades include
improved light efficiency, a larger field of view and much improved spatial
resolution.
See:
Posted by Dr. Tim Sandle, Pharmaceutical Microbiology
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