Monday, 10 August 2020

Simultaneous detection of Bacterial, Fungal and Pseudomonas aeruginosa contamination in Pharmaceutical products


Rapid detection of microbial contaminants in pharmaceutical products is play a vital role in contamination control. The purpose of this study is to standardize multiplex polymerase chain reaction (mPCR) assay to detect the pharmaceutical contaminants (either bacterial or fungal) and Pseudomonas aeruginosa specifically in single PCR reaction.

A new article of interest has been published.

A total of 13 pharmaceutical samples of ophthalmic products, chemotherapeutic, psychiatric, cardiac and gastrointestinal drugs were selected and artificially contaminated with <10 CFU of microorganisms. After enrichment, DNA was extracted, targeting the conserved region in eubacterial genome (16SrRNA), panfungal genome (28S rRNA) and P. aeruginosa (oprL). Specificity and sensitivity of the technique were verified. The mPCR assay was standardized by varying concentration of PCR reagents and conditions. In conclusion, there was no PCR inhibition and false positive results were observed. This assay can easily be incorporated for pharmaceutical products to quickly screen for microbial contamination.


The reference is:

Vijaykumar, R., Alfaiz, F. and Sandle, T. (2020) Simultaneous detection of Bacterial, Fungal and Pseudomonas aeruginosa contamination in Pharmaceutical products using Multiplex PCR Simultaneous detection of microbial contamination in pharmaceutical products using Multiplex PCR, Chimica Oggi - Chemistry Today, 38(2): 20-22

For details, please see: https://www.teknoscienze.com/chemistry-today/

Posted by Dr. Tim Sandle, Pharmaceutical Microbiology Resources (http://www.pharmamicroresources.com/)

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