Horizontal stacking effectively prevented condensation in Petri dish lids and they determined external environmental factors were not the primary cause of condensation and only had a partial influence.
An interesting study has been published in the journal Measurement Science and Technology:
Moisture condensation on the lids of Petri dishes (PDs) during incubation is a common issue in microbiological laboratories, which affects microbiological water quality testing. This research focuses on the condensation problem during the use of Reasoner’s agar (R2A) in water testing for pharmaceutical applications. Condensation can distort microbial colony counts that potentially compromise the reliability of test results. The findings presented in this work are limited to the investigated incubation conditions and R2A agar systems.
We conducted a detailed investigation into the factors contributing to condensation, particularly temperature gradients and relative humidity within vertically stacked PDs during incubation. Our experiments revealed that condensation is primarily driven by temperature differences between adjacent PDs, with higher condensation levels forming in dishes at the top of the stack. We explored various strategies, including improved air circulation and thermal insulation, but found that horizontal stacking of PDs effectively prevented condensation. These findings offer a viable method to enhance the accuracy of microbiological testing in pharmaceutical environments by mitigating moisture-related issues.
To access, see: https://www.europeanpharmaceuticalreview.com/petri-dish-study-could-enhance-accuracy-of-microbiological-testing/2135709.article
Posted by Dr. Tim Sandle, Pharmaceutical Microbiology Resources (http://www.pharmamicroresources.com/)
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