Showing posts with label Laboratory. Show all posts
Showing posts with label Laboratory. Show all posts

Sunday, 20 July 2025

What’s Inside a Bioprinter? Understanding the Machine That Prints Life

Bioprinters might look like sci-fi gadgets at first glance, but inside, they’re incredibly smart machines built to print living cells—yes, actual cells—into real tissues. If tissue engineering is the recipe, then a bioprinter is the robot chef that follows every step with surgical precision.

By Hannah Vargees

Let’s open it up (not literally, please) and see what makes it tick.

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๐Ÿ–จ️ 1. The Printhead – The Cell Dispenser  

This is the part that “writes” the tissue, one layer at a time. It’s kind of like the nozzle on an icing bag, but instead of frosting, it dispenses bioink—a mix of living cells and a gel-like material. Some printers have multiple printheads so different types of cells can be printed at once—like adding toppings to a pizza, but much more delicate.

๐Ÿงช 2. The Cartridge – Where Bioink Lives  

The cartridge is like a refillable ink tank, but for cells. It holds the bioink and keeps it safe and ready to go. Because cells are fussy little things, the cartridge is usually temperature-controlled, so they stay alive and cozy until it’s their time to shine (or rather, print).

๐ŸŽฎ 3. Movement System – The X, Y, Z Crew  

Bioprinters are all about precision. The printhead needs to move in three directions—left to right (X), front to back (Y), and up and down (Z). This movement system is like a robotic arm that knows exactly where to go and how fast, creating perfect tissue layers without a single twitch.

๐ŸŒก️ 4. Heating and Cooling Elements  

Some cells like it warm. Others prefer cool environments. That’s why bioprinters often come with heaters and chillers built into different parts—printhead, bed, or cartridge—to keep the cells in their comfort zone. Think of it as temperature-controlled room service for your cells.

๐Ÿ’ป 5. Software – The Brain Behind the Print  

Before any cell hits the surface, a 3D model of the tissue is designed on a computer. This blueprint tells the printer exactly where to deposit each drop of bioink. The software controls everything—from speed to temperature to which cell goes where. Basically, it’s the GPS, chef, and quality control manager all rolled into one.

๐Ÿ›️ 6. The Print Bed – Where It All Comes Together  

This is the surface where the tissue is built, layer by layer. It needs to be sterile, stable, and sometimes even heated to keep the structure firm and safe while it prints. You could think of it as the stage where the bioink gives its best performance.

๐Ÿ› ️ 7. Bonus Features – The Fancy Stuff  

Modern bioprinters often come with extra tools like:

  • UV curing lights – to harden certain materials

  • Cameras – to monitor printing in real time

  • Auto-calibration – so the machine adjusts itself for accuracy (because even robots need alignment sometimes)

๐Ÿ” Final Thoughts  

Bioprinters may seem complex, but each part has one simple job: to keep cells alive and print them precisely into living, functioning tissue. From the printhead to the software, every component works together like a high-tech orchestra playing the symphony of life—layer by perfect layer.

So next time you hear someone say, “They're printing skin now?!” you can nod wisely and say, “Yes. With a printhead, cartridge, and a temperature-controlled stage, of course. To know more about what bioprinters can do you can check out www.avay.tech

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Source 1  - Visit www.avay.tech to get more insights on this machine

Posted by Dr. Tim Sandle, Pharmaceutical Microbiology Resources (http://www.pharmamicroresources.com/)

Saturday, 14 June 2025

How to Audit Your Cryopreservation Workflow for Microbial Risks

 

Tubes of biological samples being placed in liquid nitrogen. Image by Dr. Vereczkey Attila CC BY-SA 3.0

Cryopreservation is an essential technology for pharmaceutical companies because it effectively preserves delicate biological samples. Storage systems are elaborate and sensitive, requiring specific temperature settings and no contaminants. Plenty of microbial risks could jeopardize everything from cells to organs, so knowing the best ways to audit workflows to eliminate these circumstances is essential.

By Emily Newton 

Review Current Floor Plans and Workflows

Workers must identify every potential contamination source by creating maps of stakeholders’ workflows. From fleet drivers to lab technicians, each step could introduce a new variable. All devices, personnel, and equipment must be reviewed at each stage to discover compliance and hygienic oversights.

 

During this stage, companies should also take stock of their most common microorganisms and discover ways to promote their survival. The facility may not be conducive, even in controlled cryovessels, to support the culture’s long-term health. This is why floor plan reviews are critical for finding bioreactor contamination situations, among other compromises. It is the reason many organizations fall short of compliance.

Mitigate Microbial Contamination Points

Observe the map and create a directory of potential contamination points. Everyone coming into contact with cryopreservation materials and the cells needs analyzing. Risks caused by human error, such as not wearing face masks or problems with the facility environment because of air quality, may also exist. Raw materials on tools and other machinery could also enter cryopreservation environments.

 

Pharmaceutical companies should perform these evaluations multiple times to understand the full scope of the workflow. For example, worker injuries may not happen every day, but a potential cut could expose specimens and transmit infection.

 

Workforces must challenge existing controls governing how to manage these samples and microbial influences and see how to improve. Staff must adequately sterilize all tools, using the most up-to-date disinfection protocols. Workers must maintain cryopreservation technologies to ensure their integrity. Personnel should also know how to document any activity regarding biological materials. If issues arise, then the source is more easily located.

Implement Microbial Testing and Observation

Most of these auditing steps concern processes before primary interaction with the microbes, ensuring temperatures range from -160 to -180 degrees Celsius. However, people working directly with biological materials must implement tests to avoid working with compromised in-process cultures.

 

Researchers should observe the quality of their clean rooms and environmental monitoring conditions, as well as the final cryopreserved product. This final observation phase could reveal unnecessary burdens or stressors on the microbes that others missed. Then, these professionals can validate the efficacy of previous quality assessments, validating if people are familiar and well-trained enough to discover something that could harm development.

Use Various Sterility Tests

Sterility testing is mandatory. Products must be free of microorganisms to preserve a drug’s effectiveness. Additionally, thorough decontamination and cleaning protocols avoid false positives when testing for contaminants, streamlining workflows further.

 

The direct inoculation method is reliable. It transfers samples into two tubes aseptically and lets them incubate at high temperatures. Membrane filtration works for cultures with properties that would not work in the conditions required for direct inoculation.

 

Experts should also ensure other protective measures are as effective as possible. Materials like dimethyl sulfoxide, which is a common cryoprotectant, are critical for preventing ice crystals in cells. Technicians should dry it out as much as possible for peak effectiveness by using one of several methods, including:

 

     Distillation from CaH2

     Molecular sieves

     Vacuum distillation

Review Data and Find Knowledge Gaps

Experts need to assess the data from workflow testing for knowledge gaps. Perfect and flawed reports alike could contain insights. Many documentation processes reveal trends in how people handle the products, reinforcing or debunking a company's quality control and mitigation strategies. The company may believe it incorporates first-in, first-out methods, but it may not be thoroughly applied in practice.

 

What was once reliable could also become inefficient. Consistently performing these tests gives a comprehensive overview of historical performance, letting people know the first instances of failures in commonly compliant areas. Deviations are the first flags in making microbes healthier among stakeholders.

Use Corrective and Preventive Actions (CAPA)

CAPA addresses the gaps analysts discover in testing reports. For example, it could include using different materials to wash workstations or minimizing contact with transportation workers. Root-cause analysis will be a continuous theme at this stage of auditing. It unravels out-of-specification results and inspires process discovery to optimize operations.

 

It could also signal an opportunity to upgrade outdated equipment that is losing durability or consistency. Many cryogenic refrigerators and other containers incorporate smart technologies, which monitor temperature changes and notify operators of dangerous anomalies. They could also automatically divert to backup power resources during an outage or other emergency to ensure essential drugs are safe.

 

Frequently, CAPA holds organizations accountable and makes them more compliant with the most essential regulations, including the Federal Drug Administration, the International Council for Harmonization and the European Medicines Agency. Many mandate testing procedures and how to establish safe working environments. It could change how facilities filter water or distribute dosages between cryogenic containers.

Microbial Quality Control

Quality assurance in pharmaceutical research is crucial for ensuring customers stay healthy. Therefore, keeping cryopreservation resources contamination-free is one of these experts' most important jobs. If they succeed, new medicines could save lives and medical facilities will execute patient recovery faster.

 

However, auditing how workers interact with microbes in cryopreserved spaces requires patience and attentiveness. Organizations must implement these practices immediately to establish good reputations and ethical practices.

 

Pharmaceutical Microbiology Resources (http://www.pharmamicroresources.com/)

Tuesday, 13 May 2025

Means, Ranges and Replicates: Improving Microbial Plate Counting


A microbial colony is a visible cluster of microorganisms growing on the surface of or within a solid medium. This may be from a single cell or an amalgam of the same organism of more than one cell or a mix of different organisms. Bioburden levels are commonly measured when conventional methods are deployed in terms of colony-forming units (CFUs). These units provide an estimation of the number of viable bacteria or fungal cells found on a sample, expressed against a unit of measurement, such as per millilitre or per milligram.

When counting CFUs on solid microbiological culture media (agar) there are some aspects of ‘counting’ that need to be considered. These are:

a) Rounding and averaging

b) Significant figures

c) Countable range

d) Statistical error from low counts

This article looks at each of these aspects of the microbial plate count.

Sandle, T. (2025) Means, Ranges and Replicates: Improving Microbial Plate Counting, European Journal of Parenteral and Pharmaceutical Sciences, 30 (1): https://www.ejpps.online/post/means-ranges-and-replicates-improving-microbial-plate-counting 

 

Posted by Dr. Tim Sandle, Pharmaceutical Microbiology Resources (http://www.pharmamicroresources.com/)

Wednesday, 19 February 2025

Scientific Methods Associating With Metrology

 Image by Greg L. CGKilogram, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=2547913

Metrology is the science of measurement and forms an excellent basis for scientific and technological advancements. That is where the application of measurement standards for diversified fields originates, with requirements of accuracy, precision, and reliability. The topic involves three main streams: scientific metrology, which is concerned with the development and maintenance of measurement standards universally; industrial metrology, which deals with the accuracy of measuring manufactured goods and their related processes; and legal metrology, which involves regulated measurements within a country for consumer protection and to promote fair trade practices. These three combined allow the various industries and countries to represent consistent, readable measurements.

Scientific Methods in Metrology

Scientific methods coupled with metrology provide the backbone and standardized tracks upon which measurement accuracies are attained and verified. One of the central aspects of metrology is defining measurement standards, such as the International System of Units. These have been upgraded to newer and more state-of-the-art research, such as the redefinition of the kilogram based on the Planck constant in 2019.

Other critical processes include calibration, which tests the precision of measuring instruments against known standards by traceability, linking all the measurements with international references. Uncertainty analysis also plays a vital role in metrology. It presents any measure's possible mistakes and limitations, turning it into a transparent and reliable one. Inter-laboratory comparisons give further validity to the measurement methods and help reach coherence among different institutions.

The Role of Metrology in Science


High-end instruments and techniques grafted onto metrology form its identity. Atomic clocks, interferometers, and spectrometers have been devised based on state-of-the-art scientific ideas promising unprecedented accuracy. Theoretical models have also been proposed to foresee the effects arising in measurement. Quantum mechanics provided a proper basis for these latest steps forward in timekeeping and electrical resistance standards. These techniques and technologies describe in detail the processes that constitute the work of metrology in science and technology. Thus, metrology is central in science.

Proper and consistent measurement is the backbone of experimental research by which scientists can study and understand many diverse aspects of nature. High-precision metrology has discovered many things in quantum physics, cosmetology, and material sciences, hence innovation in nanotechnology and biotechnology. It finds essential applications in semiconductor manufacturing, where finished products have micro-measurements.

Beyond driving innovation, however, metrology assures the quality and safety of everything medical that rely on the dependability of MRI scanners and blood pressure monitors to monitor environmental pollutants, measure climate variables, and follow the use of natural resources.

It has also led to the introduction of international cooperation. Metrology provides the basis for measurement, upon which all communication and collaboration among researchers and industry, or even governments, rests worldwide. Whether it involves vaccine development or space missions, consistency and accuracy of measurement are indispensable for the successful execution of global efforts.

Metrology supportively contributes to economic integrity through its assurance of fair trade and commerce dealing right from the much-used weighing and measuring in market transactions. The legal and regulatory systems that draw from this measurement create trust and build confidence in trade and consumer practices.

Indeed, metrology has proven to be a very important contributor to the sustainability journey toward the fight against global challenges. Precise measurements of emissions, natural resources, and environmental variation help policy and practice toward their conservation and in building climate resilience.

Written by Taylor McKnight, Author for Metrology Parts

 

Posted by Dr. Tim Sandle, Pharmaceutical Microbiology Resources (http://www.pharmamicroresources.com/)

Sunday, 30 June 2024

Experience sharing-Immunodiagnostic buffer

In the development of immunoassay reagents, buffer is typically the second most critical factor after core materials. It can affect various fundamental properties of the reagents, such as stability, sensitivity, and specificity. In previous articles, we have mentioned the roles of various buffer components several times. Because it is so crucial, today I would like to summarize it again: What are the typical components of immunoassay buffers, what materials are commonly used for each component, for everyone’s reference in the process of reagent development and optimization.

 

By Carrier Taylor 


In vitro diagnostic reagent buffers typically consist of six components: buffer agents, salt ions, stabilizers, surfactants, blockers, and preservatives. Below, we’ll elaborate on the functions of each component and common ingredients used.

Buffer agents


 

Buffer agents resist the influence of external acids or bases on solution pH, maintaining stability within a certain range. pH is a critical factor for many biochemical and enzyme-catalyzed reactions. Thus, buffer agents not only affect the stability of various proteins during storage but also ensure relatively stable pH values during reagent testing, ensuring accurate and reproducible results. Additionally, buffer agents provide a certain degree of ionic strength, promoting immune reactions and reducing nonspecific binding.

Common buffer agents include neutral PB buffer, HEPES buffer, slightly acidic MES buffer, citrate buffer, slightly alkaline Tris buffer, CB buffer, etc.

Salt ions

Salt ions in reagent buffers typically serve three functions. Firstly, they promote reaction rates. In some cases, salt ions can act as catalysts or co-factors, accelerating chemical reactions in the reagents, thereby enhancing detection sensitivity and speed. Secondly, they stabilize molecules. Salt ions can help stabilize the three-dimensional structure of proteins and other biomacromolecules through salt bridging, preventing denaturation or degradation. Lastly, they suppress nonspecific binding. In certain situations, salt ions can reduce nonspecific electrostatic interactions through charge shielding, thereby reducing false-positive results.

Common salt ions include monovalent ions such as sodium chloride, potassium chloride, and divalent ions such as magnesium chloride, calcium chloride, etc.

Stabilizers

Stabilizers, as the name suggests, maintain the stability of reagents during storage, transportation, and usage. They are typically divided into several categories. Firstly, the most common are protein stabilizers, which prevent protein and enzyme degradation and nonspecific adsorption. Secondly, sugar stabilizers. Sugars can increase stability by forming hydrogen bonds with proteins or other biological molecules, preventing denaturation or degradation. This stability is particularly important for maintaining the activity of enzymes and other biological catalysts. Lastly, other types such as glycerol, DTT, etc. Glycerol can increase solution viscosity, reducing the movement speed of protein molecules and collision frequency between molecules, which helps reduce protein denaturation. Additionally, glycerol molecules contain multiple hydroxyl groups, which can form hydrogen bonds with amino acid residues on the protein surface, stabilizing the protein’s three-dimensional structure and preventing denaturation. Glycerol can also form hydrogen bonds with water molecules, increasing effective water content in the solution, providing a stable hydration layer for proteins, thus preventing protein aggregation and precipitation. DDT is a reducing agent that can act as an antioxidant in individual projects.

Common stabilizers include protein stabilizers like BSA, BGG, casein, FSG, animal sera, sugar stabilizers like sucrose, trehalose, and others like glycerol, DTT, etc.

Surfactants

Surfactants firstly have a solubilizing effect on proteins, helping proteins maintain a stable, non-aggregated state in the buffer, which is crucial for maintaining reagent stability. Secondly, they can reduce liquid surface tension, increasing permeability, which is essential in tests where rapid penetration of reagents into samples is required. Thirdly, surfactants can assist in dispersing and suspending solid particles such as enzymes, cells, or other particles, thereby improving reagent uniformity and reaction efficiency. Additionally, surfactants can prevent nonspecific protein adsorption to container or detection device surfaces, stabilizing reagent performance. Lastly, surfactants can help improve reagent stability during storage, extending their shelf life.

Common surfactants include non-ionic surfactants like Tween, Brij-35, Triton X-100, etc.

Blockers

Blockers are divided into active and passive types, mainly aiming to reduce nonspecific binding, thereby improving reagent sensitivity and specificity. They achieve this by covering surface sites that may cause nonspecific binding, reducing background signals, making detection signals mainly derived from specific target molecules. Additionally, by reducing nonspecific adsorption, blockers can significantly improve the signal-to-noise ratio of detection signals, making the results clearer and more reliable.

Common blockers include protein blockers, polymer blockers, small molecule blockers, etc. Their main functions include anti-HAMA, anti-complement, anti-RF factor, anti-biotin interference, etc.

Preservatives

Preservatives primarily inhibit microbial growth, thereby extending product shelf life and maintaining product effectiveness. In addition to prolonging product shelf life, preservatives achieve various other objectives. For instance, microbial growth may affect reagent activity and other performance indicators. The addition of preservatives helps maintain the original performance of the reagents. By extending the shelf life of reagents, preservatives help reduce economic losses due to premature reagent disposal and increase economic benefits by increasing reagent batch size or reducing batch production.

Common preservatives include proclin, thiomersal, sodium azide, antibiotics, etc.

 

About the Author

Carrier Taylor

R & D Director and Business Development Director of BOCSCI 

 

2014 - Present, working in BOCSCI

2012-2014 Study in Rice University, MBA

2004-2008 Study in Rice University,Pharmacy 

Linkedin profile: https://www.linkedin.com/in/carrier-taylor/ 

 

Pharmaceutical Microbiology Resources (http://www.pharmamicroresources.com/)

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