How microbes eat the ocean's carbon

 Vibrio. Public Domain, https://commons.wikimedia.org/w/index.php?curid=1247054

Researchers have developed a light-emitting sugar probe that exposes how marine microbes break down complex carbohydrates. The innovative fluorescent tool allows scientists to visualize when and where sugars are degraded in the ocean. This breakthrough helps map microbial activity and carbon cycling, providing new clues about how the ocean stores and releases carbon.

Sugars and the Ocean's Carbon

Algae take in carbon dioxide and transform it into oxygen and organic matter, with sugars playing a central role in the process. Yet not every sugar is easy for microbes to digest. Some are so complex that only a few microorganisms can break them down. When these tough sugars resist decay, the carbon they contain sinks to the seafloor, where it can remain trapped for centuries until the right enzymes appear. Determining which microbes can digest specific sugars has long challenged scientists, especially in diverse ocean ecosystems.

Watching Sugars Glow

To overcome this problem, the team used automated glycan assembly to create a sugar tagged with two fluorescent dyes. These dyes interact through a process called Förster resonance energy transfer (FRET), acting together like a molecular switch. The probe stays dark when intact, but once an enzyme breaks the sugar's backbone, it emits light. This allows scientists to see exactly where and when the sugar is being degraded. The researchers tested the probe by tracking α-mannan turnover, a polysaccharide (long sugar chain) found in algal blooms. It performed effectively in purified enzyme tests, bacterial extracts, live cell cultures, and even complex microbial communities.

Revealing Hidden Degraders

By enabling the tracking of α-mannan turnover, this glycan probe opens up new avenues for studying microbial metabolism without the need for prior genomic knowledge. Researchers can now pinpoint active degraders in situ, map the progression of glycan breakdown through space and time, and quantify turnover rates in complex communities. This tool paves the way for deeper insights into glycan cycling across ecosystems, from ocean algal blooms to the human gut. By observing which microbes are activated and under what conditions, scientists can link specific enzymatic activities to environmental processes and ultimately gain a better understanding of carbon flux in the ocean.

See:

Conor J. Crawford, Greta Reintjes, Vipul Solanki, Manuel G. Ricardo, Jens Harder, Rudolf Amann, Jan-Hendrik Hehemann, Peter H. Seeberger. Activity-Based Tracking of Glycan Turnover in Microbiomes. Journal of the American Chemical Society, 2025; 147 (29): 25799 DOI: 10.1021/jacs.5c07546

 

 

 

 Posted by Dr. Tim Sandle, Pharmaceutical Microbiology Resources (http://www.pharmamicroresources.com/)