Here
is the abstract:
“Antibiotic
resistance is arguably the most serious health-related issue of the current
time. This is even more so with carbapenem-resistant Enterobacteriaceae, for
such microorganisms are resistant to the carbapenems (the ‘antibiotics of last
resort’). One of the most important considerations is in the detection of
bacteria that carry the carbapenem-resistant gene. For this, molecular-based
phenotypic and genetic-based polymerase chain reaction (PCR) methods are
available. In contrast to phenotypic methods, molecular-genetic techniques,
such as PCR, are considered to have the potential for improved detection of
carbapenem-resistant genes by virtue of specificity, accuracy and rapidity. The
tendency in PCR techniques is to develop towards the real-time systems equipped
with multiplexing functionality. However, as shown in our study, standard PCR
with electrophoresis detection continues widely to be used for the detection
and identification of the carbapenemase gene. Therefore, despite progress in PCR
technology, methods deployed for the detection of serious hospital acquired
infections around the world are arguably neither the most accurate nor the most
efficient. This issue is of concern for pharmaceutical scientists in relation
to the use and development of PCR technology and in relation to new drug
development.”
The
reference is:
Sandle,
T., Banenko, D., Lavrinenko, A., Azizov, I. and Chesca, A. (2014) The current
state of PCR approach in detection and identification of carbapanem hydrolysis
β-lactamases genes, European Journal of
Parenteral and Pharmaceutical Sciences, 19 (1): 153-164
Posted by Tim Sandle
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