Tuesday 17 June 2014

PCR for detection and identification of carbapanem hydrolysis β-lactamases genes

A new paper of interest has been published, titled: “The current state of PCR approach in detection and identification of carbapanem hydrolysis β-lactamases genes”. The paper has been written by Tim Sandle, Dmitriy Babenko, Alena Lavrinenko, Ilya Azizov and Antonella Cheșcă (Pharmig, Karaganda State Medical University and University of Brașov).

Here is the abstract:

“Antibiotic resistance is arguably the most serious health-related issue of the current time. This is even more so with carbapenem-resistant Enterobacteriaceae, for such microorganisms are resistant to the carbapenems (the ‘antibiotics of last resort’). One of the most important considerations is in the detection of bacteria that carry the carbapenem-resistant gene. For this, molecular-based phenotypic and genetic-based polymerase chain reaction (PCR) methods are available. In contrast to phenotypic methods, molecular-genetic techniques, such as PCR, are considered to have the potential for improved detection of carbapenem-resistant genes by virtue of specificity, accuracy and rapidity. The tendency in PCR techniques is to develop towards the real-time systems equipped with multiplexing functionality. However, as shown in our study, standard PCR with electrophoresis detection continues widely to be used for the detection and identification of the carbapenemase gene. Therefore, despite progress in PCR technology, methods deployed for the detection of serious hospital acquired infections around the world are arguably neither the most accurate nor the most efficient. This issue is of concern for pharmaceutical scientists in relation to the use and development of PCR technology and in relation to new drug development.”

The reference is:

Sandle, T., Banenko, D., Lavrinenko, A., Azizov, I. and Chesca, A. (2014) The current state of PCR approach in detection and identification of carbapanem hydrolysis β-lactamases genes, European Journal of Parenteral and Pharmaceutical Sciences, 19 (1): 153-164

To obtain a copy, please contact Tim Sandle

Posted by Tim Sandle

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