Pipetting
accuracy and precision are the key factors for successful experiments in the laboratory.
The best pipetting performance is achieved, when the pipette and the tip match
perfectly with each other, and the tip being used is optimal for the liquid
being transferred.
When
pipetting liquids with low surface tension (e.g. detergents), they tend to
leave a film of liquid on the inner surface of standard polypropylene pipette
tips, leading to inconsistency and inaccuracy in pipetting as well as loss in
volume of valuable samples.
Various
technologies are being used to modify the standard pipette tips to mitigate
this issue. This application note describes the comparison of various
manufacturers’ low retention tips, when handling liquids that contain
detergents. The results show that Sartorius Low Retention Tips ensure near
complete recovery of the sample, when pipetting liquids of low surface tension.
When comparing to other low retention tips from various manufacturers,
Sartorius tips secured the best sample recovery and the best chemical
resistance.
Posted by Dr. Tim Sandle
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