Researchers in Spain are using RPA as the foundation for a highly sensitive and specific solid-phase optical assay that can detect the potential biowarfare agent, Y. pestis, in less than an hour. The enzyme-linked oligonucleotide assay (ELONA) approach developed by Ioanis Katakis and Ciara K. O’Sullivan, at the Universitat Rovira i Virgili’s Interfibio Research Group, and the ICREA (Catalan Institution for Research and Advanced Studies), uses conventional PCR primers to amplify both single- and double-stranded Y. pestis DNA. Their work provides proof of concept for applying RPA in a heterogeneous format, with one primer immobilized onto a solid surface. The researchers aim to further develop the technology into an integrated, portable lateral flow-type test device for the rapid amplification and detection of Y. pestis in resource limited and field settings.
For further details see:
![](//ws-na.amazon-adsystem.com/widgets/q?_encoding=UTF8&ASIN=B01EIG23MY&Format=_SL250_&ID=AsinImage&MarketPlace=US&ServiceVersion=20070822&WS=1&tag=pharmigpharma-20)
![](https://ir-na.amazon-adsystem.com/e/ir?t=pharmigpharma-20&l=li3&o=1&a=B01EIG23MY)
For further details see:
No comments:
Post a Comment
Pharmaceutical Microbiology Resources