Tuesday, 30 May 2017

How Gonorrhea manipulates the female reproductive tract


Gonorrhea is a widespread sexually transmitted disease caused when Neisseria gonorrhoeae bacteria infect the normally protective inner lining of human genital tissues. In women, the opening of the uterus, known as the endocervix, serves as a primary infection site for N. gonorrhoeae. However, the strategy used by N. gonorrhoeae to penetrate the lining of the endocervix has been unclear.

To investigate this mechanism, Liang-Chun Wang of the University of Maryland, College Park, and colleagues needed to develop an alternative to the mouse models normally used to study gonorrhea, since they have been inadequate for this purpose. The team developed a new model using tissue samples obtained from the human endocervix.

The researchers infected the endocervix tissue, as well as lab-grown cells of the same type as those that line the endocervix, with N. gonorrhoeae. They then employed a variety of molecular and imaging techniques to examine the infection mechanism.

The results demonstrate that N. gonorrhoeae penetrates the endocervix lining by interfering with a normally protective process. Usually, infected cells in the lining can be shed and disposed of without breaking the tight connections between cells that keep the lining uncompromised. N. gonorrhoeae appears to be able to break these connections and induce cell shedding, opening paths for penetration without reducing its ability to adhere to and invade the cells of the lining.

See:

Liang-Chun Wang, Qian Yu, Vonetta Edwards, Brian Lin, Jessica Qiu, Jerrold R. Turner, Daniel C. Stein, Wenxia Song. Neisseria gonorrhoeae infects the human endocervix by activating non-muscle myosin II-mediated epithelial exfoliation. PLOS Pathogens, 2017; 13 (4): e1006269 DOI: 10.1371/journal.ppat.1006269

Posted by Dr. Tim Sandle

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